| abstract |
Provided is an HEK293 cell-based glutamine synthetase-deficient cell line HEK293-GS-/- that can be stably passaged, adapted to a suspension culture, and is applicable to recombinant protein expression constructed using a CRISPR/Cas9 system. The cell line can express various recombinant proteins through screening using a GS/MSX screening system, can stably express a target protein after multiple passages, and can adapt to most commercial serum-free media. |