abstract |
Nucleic acid constructs encoding a chimeric antigen receptor (CAR) and a truncated human epidermal growth factor receptor (huEGFRt) are described. The encoded CARs include a tumor antigen- specific monoclonal antibody, such as a glypican-3 (GPC3)-specific, a GPC2-specific or a mesothelin- specific monoclonal antibody, fused to a CD8α hinge region, a CD8α transmembrane region, a 4-1BB co- stimulatory domain and a CD3ζ signaling domain. Isolated host cells, such as isolated T cells that co- express the disclosed CARs and huEGFRt are also described. T cells transduced with the disclosed CAR constructs can be used for cancer immunotherapy. |