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publicationDate 2019-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2019087393-A1
titleOfInvention Component separation method using supercritical fluid chromatograph
abstract For the separation of a peptide or protein in a sample using a supercritical fluid chromatograph, carbon dioxide is used as the supercritical fluid, while an organic solvent containing hexafluoroisopropanol is used as the modifier to be added to the supercritical fluid. If a mixed liquid of methanol, isopropanol, water and formic acid is simply used as the modifier, a considerable amount of peak tailing occurs on the chromatogram, causing the peak waveform to be distorted. By comparison, when a mixed liquid prepared by additionally mixing hexafluoroisopropanol in the mixed liquid of methanol, isopropanol, water and formic acid is used, the peak observed on the chromatogram will have a sharp, satisfactory shape. Thus, it becomes possible to perform an analysis of a peptide with high sensitivity and high accuracy, or a purification of a peptide to a high degree of purity, using a supercritical fluid chromatograph.
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