| abstract |
PROBLEM TO BE SOLVED: To provide a culture method and the like that can keep the viscosity of a culture solution low during liquid culture and at the end of culture of Rhizobium spp., So that subsequent concentration and viable cell separation become easy and efficient. . SOLUTION: A medium is used in which disaccharides (eg, maltose, trehalose, lactose, sucrose, etc.) account for 25% by mass or more of carbon sources in the medium, and the pH of the culture solution is from the start of culture to the end of culture The above-mentioned problems are solved by carrying out liquid culture of Rhizobium spp. While maintaining it in the range of 5.0 to 7.0. |