http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019077056-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e2817954cd008bcd609a4c4eb49050be |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-0203 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-44 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
filingDate | 2018-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ce0e0f122dcfa4a6d811c9eb663f1eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aabc8d0594bfc7ebe141c4ecaa632680 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_104ec3e831eaa90760e6cfe7ae65699a |
publicationDate | 2019-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2019077056-A1 |
titleOfInvention | MEANS AND METHODS FOR PRODUCING ADP-RIBOSYLATED FORMS OF PROTEINS AND PEPTIDES |
abstract | The present invention relates to a method for producing an ADP-ribosylated serine protein or peptide comprising preparing an aqueous buffered solution comprising from 5 to 60 mM, preferably from 10 to 60 mM of a buffer and having a pH of from 5.0 to 9.0, preferably from 5.5 to 8.5, and most preferably from 6.1 to 8.3, said solution further comprising (a) 0.2 to 2.5 mM NAD + , (b) at least 50 nM, preferably 50 to 3000 nM PARP-1, PARP-2 or the PARP-1 variant E988Q, (c) at least 100 nM, preferably 100 to 5000 nM of HPF1, (d) at least 10 μg / mL, preferably 10 μg / mL to 200 μg / mL of sonicated DNA, said sonicated DNA preferably comprising DNA fragments of 10 to 330 bp, and (e) up to 600 μΜ of protein or peptide, said protein or peptide comprising at least one serine, thereby generating a reaction mixture, wherein the protein or peptide becomes an ADP-ribosylated serine. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111721939-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111721939-B |
priorityDate | 2017-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 144.