http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019051703-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b946f8a160a77d9f664816a704364d83 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y115-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0089 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
| filingDate | 2017-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91ae5d9d47d70dd6c13bd12c2f394e5d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae9be4524eb7f54fee702be768fa2d42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af9ab1b0b6ac99bc09ab1d5f9c1ddfd2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8619399d362af0a30e1d74af98ad6d8e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc0861336b18060160be44a83af9a090 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9c33c5b5b5a856dec9fbe544b2a38a1 |
| publicationDate | 2019-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2019051703-A1 |
| titleOfInvention | PROCESS FOR RECOVERING ACTIVITY OF SUPEROXIDE DISMUTASE TO COPPER AND HUMAN RECOMBINANT ZINC |
| abstract | The invention belongs to the field of protein medicine bioengineering and technology, and discloses a method for recovering the activity of recombinant human copper zinc superoxide dismutase, and the SOD protein is in a stretch state and then a solution containing Cu 2+ and β-mercaptoethanol are added. The method of the invention makes the protein in a stretch state by changing the environment of the protein, and then adding copper ions to fully react to form a correct coordination bond, thereby obtaining the activity of the protein, and adding a free sulfhydryl group of the modified amino acid of β-mercaptoethanol to make the protein molecule The correct disulfide bond is formed to form a structurally stable Cu, Zn-SOD. It has been proved by experiments that Cu, Zn-SOD can be treated by the method of the invention, Cu, Zn-SOD can effectively restore protein activity and the structure is stable and uniform. |
| priorityDate | 2017-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 105.