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filingDate 2018-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99ae368dff7404a8934a2cdf0b5fe044
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publicationDate 2019-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2019050033-A1
titleOfInvention Bacillus subtilis thermophilic bacterium-derived mutant glycine oxidase and method for producing the same
abstract The invention provides a mutant glycine oxidase comprising an amino acid sequence produced by substitution of at least one amino acid residue in the wild-type amino acid sequence derived from a thermophilic bacterium belonging to the Bacillus family by another amino acid residue, which has the following enzymatic properties: molecular weight: 40,000 ± 2,000 daltons as measured by SDS-PAGE; optimum temperature: 45 ° C as measured in the presence of a salt of pyrophosphoric acid under conditions of pH 8.5; Optimum pH: pH 8.0 as measured in the presence of a salt of pyrophosphoric acid under the condition of 37 ° C; thermal stability: stable at a temperature up to 70 ° C as measured in the presence of a salt of pyrophosphoric acid under conditions of pH 8.5 maintained for 1 hour; pH stability: stable at a pH value in the range of 5.5 to 10.0 as measured in the presence of a pyrophosphoric acid salt under the condition of 4 ° C maintained for 24 hours; specific activity: 1.2 units / mg or more; and a kinetic constant K m : 0.2 mM or less.
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