| abstract |
The present invention relates to methods for modifying the genome of a fungal cell, comprising the steps of: (A) introducing into the fungal cell: (i) a first nucleic acid construct comprising a polynucleotide encoding an endonuclease of RNA-guided DNA; (ii) a second nucleic acid construct comprising (a) a U6 promoter sequence operably linked to (1) a transfer RNA coding sequence, (2) a single guide RNA coding sequence, and (b) a termination sequence U6; and optionally (iii) a third nucleic acid construct comprising a donor DNA for modifying the genome; and (B) selecting a transformant of the fungal cell, the genome being modified. |