http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019005978-A3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0ca0841c52dc980c3f201762d410f79b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_df397e98b135735bd429456a0e38ed3d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1082 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6855 |
| filingDate | 2018-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0029dc8cd1e1b45d65fc3428b811f80 |
| publicationDate | 2019-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2019005978-A3 |
| titleOfInvention | METHODS OF SUPPRESSING FORMATION OF ADAPTER DIMERS IN DEEP SEQUENCING LIBRARY PREPARATION |
| abstract | The present invention provides methods of suppressing the formation of adapter dimers comprising: providing a target polynucleotide having a 5 'end and a 3' end; the use of a double-stranded DNA adapter with a 5 'end and a 3' end which have complementary sequences from each other, ligation of the double-stranded adapter to the target polynucleotide to form a ligation product . The invention also relates to a method for preparing a library of nucleic acid sequences comprising: providing a double-stranded DNA adapter with 5 'and 3' ends having sequences complementary to each other contacting the adapter with target nucleic acid sequences having a 5 'end and a 3' end, and ligating the adapter with a sequence complementary to the 5 'and 3' ends of the target nucleic acid using a double-stranded DNA ligase. The invention also relates to kits for suppressing adapter dimer formation in deep sequencing containing a double-stranded DNA adapter with 5 'and 3' ends having sequences complementary to one another, appropriate enzymes, buffers, dNTPS, etc. |
| priorityDate | 2017-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 21.