| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eacc790850fbe5bd5ad30c690278a033 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-173
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2561-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-537 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6846 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 |
| filingDate |
2017-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59235d28ff55482c75a2398fc20f293e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aeb26aab8a690178152b0b964ab9595b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e5769eb3ec133fc17d959e796b8f817
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ee0ed90d523276f4ef78742cc38b7ed
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ad4bd9ff7a0d1b0e5a2b9131c545570
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f504dce42b25d1b7a1934bce41e2d04e |
| publicationDate |
2018-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2018235110-A1 |
| titleOfInvention |
METHODS FOR DETECTION OF TARGET DNA DIRECTLY IN RAW SAMPLES BY POLYMERASE CHAIN REACTION AND GENOTYPING THROUGH HIGH RESOLUTION FUSION ANALYSIS |
| abstract |
The invention relates to a method for detecting target DNA directly in raw samples by polymerase chain reaction (PCR) and genotyping via high resolution fusion analysis (HRM). The method consists in diluting the raw sample, treating the diluted crude sample by passing it through a ramp for increasing and then decreasing the temperature, performing PCR amplification and then HRM analysis. The method further includes monitoring, during the HRM analysis, the variation of the signal emission resulting from the temperature-induced denaturation of the double-stranded amplicons into two single-stranded DNAs, due to the release of a molecule or an intercalation compound. The detection of target DNAs in the raw sample is carried out via a reader analyzing the variation of the signal, obtaining the result of the analysis by means of a graphical interface connected to the reader. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021180337-A1 |
| priorityDate |
2017-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |