| abstract |
The present invention relates to an enzymatic composition and the development of an enzymatic cascade for the production of 2,5-furanodicarboxylic acid (FDCA) from 5-methoxymethylfurfural (MMF). The cascade is triggered by oxidation of MMF by aryl alcohol oxidase, which produces H 2 O 2 so that the non-specific peroxygenase cleaves an ether bond, thereby releasing methanol, and subsequent oxygenation, which leads to the production of FDCA. Another enzyme, namely methanol oxidase, was selected for in situ production of the additional H 2 O 2 required by the non-specific peroxidase by oxidation of a reaction byproduct. |