http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018227665-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_75d25c983d4c07d8cdd348924f2f5c76 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-795 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-795 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 |
| filingDate | 2017-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bcbbd0bef9eedf602889cf1c29c69d74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_676ae215a93810f58848c2aa7eedecca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0de98e5264380d7ca746d5104ae8a7e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1cdad7bb8f6bf0258e0771a63274682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_473c8631c47fa5f19f596201ad6b1091 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f949c80ea794c619980339a4f8fe2e55 |
| publicationDate | 2018-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2018227665-A1 |
| titleOfInvention | Methods for extracting and purifying nostoc sphaeroides kutzing phycobiliprotein, and purified phycocyanin |
| abstract | Provided is a method for purifying a Nostoc sphaeroides Kutzing phycocyanin, the method comprising the following steps: (1) selecting an anion exchange column for being passed through ; (2) formulating a Tris-HCl buffer solution, i.e., buffer A, and a Tris-HCl-NaCl buffer solution, i.e., buffer B; (3) dissolving a coarsely extracted Nostoc sphaeroides Kutzing phycobiliprotein powder with buffer A, and subjecting same to centrifugation and filtration; (4) washing a pump, and balancing the ion exchange column with the buffer A buffer solution; (5) directly loading the Nostoc sphaeroides Kutzing phycobiliprotein dissolved in buffer A and prepared in step (3), carrying out gradient elution with buffer B, and collecting an eluent; (6) cleaning the ion exchange column with a salt solution, and then reversely rinsing same with a base solution; and (7) subjecting the eluent to ultrafiltration concentration and dialysis to obtain a purified Nostoc sphaeroides Kutzing phycocyanin aqueous solution. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109757648-A |
| priorityDate | 2017-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.