| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c8ed020d9d1eed3acfa2285b2f55de30 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 |
| filingDate |
2018-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ab059000b4d773e4aec9c890d0ec5d0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31236b995dd24f8cf4a409fc9d5754c5 |
| publicationDate |
2018-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2018203056-A1 |
| titleOfInvention |
Dna assembly |
| abstract |
The invention relates to a nucleic acid for use in DNA assembly, wherein the nucleic acid comprises at least one methylation-protectable restriction element, the methylation-protectable restriction element comprising: a restriction enzyme recognition sequence that is recognised by a restriction enzyme that cleaves outside of the recognition sequence; and a DNA methylase recognition sequence, wherein the restriction enzyme recognition sequence and the DNA methylase recognition sequence overlap such that the base modified by the DNA methylase lies within the restriction enzyme recognition sequence, wherein the DNA methylase recognition sequence is not identical to or enclosed by the restriction enzyme recognition sequence, and wherein the DNA methylase recognition sequence does not overlap with the sequence that would form the overhang end sequence generated by the restriction enzyme. The invention further relates to asscociated methods and kits. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3908666-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021192596-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020201434-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-201904653-D0
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113544274-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023187175-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7332699-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2022522397-A |
| priorityDate |
2017-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |