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publicationDate 2018-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2018178920-A1
titleOfInvention A rapid, on-demand heparin-induced thrombocytopenia functional assay
abstract A method for assaying a heparin-induced thrombocytopenia (HIT) in a patient's serum or plasma sample, the method comprising: incubating a mix of platelets with the patient's serum or plasma sample in the presence of either a low concentration of heparin or a high concentration of heparin; incubating a mix of platelets in the presence or absence of a platelet activator; quantifying platelets and activated platelets in the mixes; calculating percentage of activated platelets within the platelets for each of the mixes; calculating a heparin platelet activation (HEPLA) index using the calculated percentages; measuring and calculating HEPLA indices of serum or plasma samples from donors not suffering from HIT; calculating cut-off values from the HEPLA indices of serum or plasma samples from donors not suffering from HIT, and determining whether a patient suffers of HIT or not by comparing the HEPLA index of the patient with the cut-off values.
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