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publicationDate 2018-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2018175424-A1
titleOfInvention System for rapid, portable, and multiplexed detection and identification of pathogen specific nucleic acid sequences
abstract A sample carrier may include a sample preparation module and an amplication module. A sample mixes with a lysis medium and a nucleic acid amplification medium in the sample preparation module and then flows into a plurality of mierofiuidie chambers in the amplification module. The microfhiidic chambers have disposed therein primers configured to initiate amplification of one or more target nucleic acid sequences corresponding to one or more pathogens. The sample carrier is inserted into an apparatus that includes a plurality of Sight sources and a camera. The light sources illuminate ihe mierofiuidie chambers with excitation light, a f!uorophore emits il.uorescenee light indicative of nucleic acid amplification in response to the excitation -light, and ihe camera captures images of the mierofiuidie chambers. A target nucleic acid sequence in the sample is indicated by the images showing an increasing fluorescence in a microfluidie chamber that has the primers for that sequence.
priorityDate 2017-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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