| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16C20-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B20-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B20-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1089
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1068
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6811
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B35-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B20-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B20-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B30-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B35-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B20-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B20-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B20-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6811
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-02 |
| filingDate |
2018-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba20b4d5e0fba89440b0043f70d4cb54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_765a871db3ba55b10e439f2bf57ec35e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d1703b910a6e79801b81f54e6e698d7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e9e30f7815dd564accebf948c05c22f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_365f86024080a68eeb82212b3be740d7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad76a39391ccb6f1cb23d412c744f8c8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d4b47555138661c1b55fbcc485b06bb |
| publicationDate |
2018-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2018162376-A1 |
| titleOfInvention |
Method for discovery of alternative antigen specific antibody variants |
| abstract |
Herein is reported a method for selecting a variant of a parental antibody variable domain encoding nucleic acid, wherein the parental antibody variable domain amino acid sequence encoded by said encoding nucleic acid has at least one developability hot spot, the method comprising the steps of (i) providing a multitude of DNA-containing samples (genomic material of antibody secreting B- cell) each including one or more antibody variable domain encoding nucleic acids; (ii) performing PCR amplification of said antibody variable domain encoding nucleic acids of (i) using consensus sequence-specific primers to obtain amplification products (wherein said consensus sequence-specific primers bind to consensus sequences that are common to a plurality of genes within the genetic loci set, thereby generating a pool of amplification products); (iii) sequencing a plurality of said amplification products obtained in step (ii) in order to determine the relative proportion of each nucleotide at each position in a sequencing read; (iv) performing a sequence alignment between the sequencing read results of (iii) and the parental antibody variable domain encoding nucleic acid; (v) performing a sequence-identity/homology-based ranking of the antibody variable domain encoding nucleic acids in said sequence alignment with the parental antibody variable domain encoding nucleic acid being the perfect/template/reference sequence; and (vi) selecting the variant antibody variable domain encoding nucleic acid based on the sequence ranking of step (v), whereby the variant selected in step (vi) is selected so that the developability hot-spot is removed. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020213730-A1 |
| priorityDate |
2017-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |