| abstract |
In alternative embodiments, provided are compositions, including products of manufacture and kits, and methods, for remotely-controlled and non-invasive manipulation of genetic processes in live cells, e.g., for monitoring physiologic processes, for the correction or treatment of pathological processes and for control of therapeutic outcomes. In alternative embodiments, provided are ultrasound-based mechanical stimulations and a mechano-sensitive protein, e.g., a transmembrane protein or a channel or channels, either synthetically engineered or natively (endogenously) occurring, integrated to control the production of nucleic acids, genes and/or biological-active proteins, which can be used, in alternative embodiments, for diagnostic or therapeutic purposes. In alternative embodiments, exemplary mechanogenetic systems provided herein, being based on ultrasound, allow a deep penetration of stimulation and manipulation in vivo at centimeter-level depth with high spatiotemporal precision. |