| abstract |
The invention provides processes for producing or propagating a formative stem (FS) cell line, from one or more precursor pluripotent stem cells, which process comprises: (a) providing a one or more precursor pluripotent stem cells; (b) culturing the precursor pluripotent stem cells in formative stem cell culture media. The formative stem cell culture media comprises limited amounts of activin and exogenous fibroblast growth factor, but includes a Wnt inhibitor, and typically a retinoic acid receptor inhibitor. The invention further provides related materials and methods for use in preparing and utilising FS cell lines. |