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filingDate 2018-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2018-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2018136881-A1
titleOfInvention Sequencing adapter manufacture and use
abstract Technology provided herein relates in part to methods, processes, machines and apparatuses for determining sequences of nucleotides for nucleic acid templates in a nucleic acid sample. The technology provide herein also relates in part to methods, processes, machines and apparatuses for counting nucleic acid templates. Nucleic acid templates of a sample are tagged with nonrandom oligonucleotide adapters that include predetermined non-randomly generated sequences. The use of these nonrandom oligonucleotide adapters provides an efficient method to reduce sequencing errors, and increase the sensitivity of detection of low-frequency single nucleotide alterations.
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