| abstract |
The present invention relates to methods for engineering yeast strains which can be used in the production of industrially important chemicals. Nucleic acids encoding components of the methanol utilization pathway are used to modify yeast cells such as Saccharomyces cerevisiae to enable them to grow in media in which methanol is the only or main energy source. The nucleic acids may encode proteins including alcohol oxidase, dihydroxyacetone synthase, dihydroxyacetone kinase, fructose- 1-6-bisphosphate aldolase, fructose-1-6-bisphosphatase, sedoheptulose bisphosphatase, ribose-5-phosphate isomerase, D-ribulose-5-phosphate-3-epimerase, transketolase 1 or 2, transaldolase and formaldehyde dehydrogenase. |