| abstract |
Provided is a high-loading alkali-resistant protein A magnetic bead. The magnetic bead can maintain chemical stability under pH 2-14 and has an immunoglobulin G (IgG) binding capacity of at least 50 mg/mL. Further provided is a method for purifying and/or detecting an immunoglobulin, comprising a step in which a sample containing the immunoglobulin contacts the high-loading and alkali-resistant protein A magnetic bead. The alkali-resistant protein A magnetic bead can realize rapid purification of immunoglobulin, saving about 80% of treatment time and reducing purification costs by 50%. The alkali-resistant protein A magnetic bead has high alkali resistance. An alkaline method for in situ purification can be performed to regenerate the magnetic bead after use. The magnetic bead has rapid magnetic response and good dispersiveness, realizing rapid magnetic bead enrichment, cleaning, and elution. The magnetic bead facilitates automated, high-throughput, and large volume purification of a sample. |