Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_45ba2a5f77de88b615dcfad1864ebb90 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1062 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1081 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-2893 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1018 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-4088 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-302 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-49 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N35-00623 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-1012 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0641 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0644 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-49 |
filingDate |
2017-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c81daecf61cf2fe77df4713a235a9ebc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a12b81f386f9f3760b56fb6cb9ba2f0b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2bee94376feabb326872487a0dee17c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3752a4866f6b8e9f15eb3c439cc5e448 |
publicationDate |
2018-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2018126499-A1 |
titleOfInvention |
Method for preparing reticulocyte simulated particle and platelet simulated particle, and quality control material |
abstract |
Provided are a method for preparing reticulocyte simulated particles and platelet simulated particles, and a quality control material. The method for preparing the reticulocyte simulated particles uses an N-hydroxysuccinimide-activated protein fluorescent dye to stain mammalian enucleated red blood cells with a volume of 60-120 fL and performs the fixed treatment to the enucleated red blood cells to prepare reticulocyte simulated particles. The platelet simulated particles are prepared using mammalian enucleated red blood cells with a volume of 2-25 fL, and the rest is the same as that for the reticulocyte simulated particles. The preparation method comprises: using an N-hydroxysuccinimide-activated protein fluorescent dye to stain different volumes of mammalian enucleated red blood cells, and obtaining reticulocyte simulated particles and platelet simulated particles respectively. The scatter diagram fluorescence and volume direction thereof are close to the scatter diagram distribution of reticulocytes, reticulated platelets, and platelets in fresh blood. The prepared simulated particles have good stability and do not interfere with the count classification of other cells and particles. |
priorityDate |
2017-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |