| abstract |
The present invention provides a D-psicose-3-epimerase mutant for efficient and effective production of D-allulose (also known as D-psicose) from D- fructose. The invention represents an advancement in the field of enzyme engineering. The mutant enzyme has increased thermo-stability, operational stability and is functional at wide range of pH as compared to enzymes existing in the prior art and can be used for obtaining high yield of D-allulose with minimum downstream processing cost. Further, the invention relates to nucleic acid encoding the said protein, expression cassettes, vectors, recombinant host cells for preparing the mutant enzyme and processes for preparing the mutant enzyme and processes for production of D-allulose from D-fructose using the mutant enzyme. |