| abstract |
Provided in the present invention is a cell freezing medium for clinical use. In particular, the cell freezing medium of the present invention comprises the following components: (1) human albumin; (2) a cryoprotectant, wherein the cryoprotectant comprises a combination of one or more of dimethyl sulfoxide, glycerol and ethylene glycol; (3) a saline buffer, wherein the saline buffer is a solution comprising ions Na+, K+, Mg+, Cl- and CH3COO-; (4) a vitamin; and (5) an amino acid, wherein the concentration of the human albumin is 1-20% (w/v). The cell, after long-term cryopreservation with the freezing medium of the present invention, has a high viability, and the cellular efficiency maintains a high uniformity. The grade of purity of the freezing medium of the present invention is the pharmaceutical grade or USP grade; and the freezing medium is safe and reliable for clinical use, and can be used for conventional adherent and suspension cells. |