| abstract |
A multilayer microfluidic device for cell culture in the gradient of a bioactive substance characterised by three layers and two protective cups (3) of tanks, the top layer being a cover (6), which contains tanks of fluids feeding the culture chambers (1), reference points (4) and a millimetre graduation (5); in the middle functional layer (7), made of adhesive film, there is a set of channels serving as culture and indicator chambers (2) with semi-circular ends (8), at least one of which is intended for cell culture in concentration gradient of the active substance (culture chamber), one for cell culture without an active agent (blind test) and one to develop a concentration profile of the indicator substance (indicator chamber); and the bottom layer is the base (9). |