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publicationDate 2018-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2018045014-A1
titleOfInvention Stimulated emission depletion nonlinear structured illumination microscopy (sted-nsim) apparatus, methods, and applications
abstract A superresolution STED-NSEVI apparatus having an epifluorescence architecture utilizing a 2D structured STED pattern having a N.A. less than a N.A. of the microscope objective and no surface plasmon resonance (SPR) effects. A superresolution STED-NSEVI imaging method using a fully deterministic imaging processing method, in which a pre- calibrated set of parameters are used to process all image data.
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