| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_74b7b50944d8e1960440fb1f5440b7fd |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D2311-2626
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D2315-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D2311-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D61-145 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-1871
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-362
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-327
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D61-145
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 |
| filingDate |
2017-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83185203854644dc2a28491269f7a5be
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_543186b632b3f99390c3eb76b2f36e50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2af4da7f06768a3792383e04c9c172e9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_161e4418aa888898e9365104995be31a |
| publicationDate |
2018-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2018039163-A1 |
| titleOfInvention |
Method of purifying a heterologous protein from an egg white |
| abstract |
The present invention is related a method of purifying a heterologous protein from an egg white. In some embodiments, the disclosure is directed to a method of purifying a heterologous protein from an egg white comprising the heterologous protein, the method comprising, (a) adjusting the pH of the egg white to a pH of 5.8 to 6.5 to form a pH-adjusted egg white; (b) filtering the pH-adjusted egg white of (a) and collecting a first filtrate; (c) subjecting the first filtrate of (b) to a hydrophobic interaction chromatography matrix, and collecting a first eluate comprising the heterologous protein; (d) adjusting the pH of the first eluate of (c) to a pH of 5.0 to 5.6 to form a pH-adjusted eluate; (e) filtering the pH-adjusted eluate to obtain a second filtrate; (f) adjusting the pH of the second filtrate to a pH of 3.0 to 4.0 to form a pH-adjusted second filtrate; (g) neutralizing the pH-adjusted second filtrate of (f) to a pH of 5.0 to 8.0 to form a neutralized solution; (h) subjecting the neutralized solution to a cation exchange chromatography matrix and collecting a second eluate comprising the heterologous protein. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2022511044-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020115196-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3133932-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102565593-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20210074390-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102018009597-A1 |
| priorityDate |
2016-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |