http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018023165-A1

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filingDate 2017-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2018-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2018023165-A1
titleOfInvention A method for distinguishing between more than one fluorescent species present in a sample
abstract Methods and systems are provided for distinguishing between more than one fluorescent species present in a sample in fluorescence microscopy. The method involves illuminating the sample with at least one light source. More than two images of the illuminated sample are recorded over a period of time, each image comprising a plurality of pixels, wherein each pixel corresponds to a location in the sample and records a degree of fluorescence at the location in the sample at a particular point in time. A photostability characteristic of the degree of fluorescence at each pixel over the period of time over which the more than two images were recorded is determined and used to distinguish between the more than one fluorescent species present in the sample.
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