| abstract |
The present invention provides a fermentation technique to improve the production level of a recombinant human collagen. First inoculating a sterilized fermentation culture medium with a Pichia yeast solution, then performing fermentation culturing for 14-18 hours before adding methanol to induce the expression, and adding sodium pyruvate into the culture medium, wherein the amount of the sodium pyruvate added is 0.01-10 g/L. |