| abstract |
The present invention generally relates to systems and methods for determining RNA in blood or other fluids. In certain embodiments, blood or other fluids may be treated to isolate or separate RNA, for example, from DNA, cells, and other material. In some cases, the RNA may arise from bacteria or other pathogens or foreign organisms that may be found within the blood or other fluid. In some cases, RNA stabilizing reagents, such as ammonium sulfate, may be added to stabilize RNA, then cells within the blood may be lysed to release the RNA (and other materials) from the cells, thereby producing a lysate. The lysate may be treated, e.g., to separate nucleic acids from other components within the lysate, and in some cases, DNA may be degraded, e.g., using DNAses or other suitable enzymes, leaving behind the RNA. The RNA can then be studied, purified, analyzed, amplified, stored, or the like. |