| abstract |
A method for producing a cell population comprising mast cells, the method comprising, a) providing a population of cells capable of differentiation into mast cells, the cells comprising a selectable reporter linked to Gata2 expression levels in said cells, b) culturing said cells under conditions adapted to promote hematopoietic cell development, c) isolating cells expressing Gata2, d) culturing said isolated cells on feeder cells; thereby obtaining a cell population comprising mast cells. The invention also relates to a population of cells comprising mast cells made according to the above method, and a nucleic acid sequence used in the aforementioned method. |