| abstract |
The present application provides a system, composition, method and kit for removing a detergent, such as an anionic detergent, from an aqueous solution, comprising a salt, a water immiscible alcohol of Formula I: R 1 – OH (Formula I) where R1 is an optionally substituted, linear, branched or cyclic C 4 - C 12 alkyl; and a water immiscible halocarbon, wherein said halocarbon is miscible with said alcohol of Formula I. The system can be used on aqueous solutions that contain detergents (such as Sodium Dodecyl Sulphate (SDS), for example), and any detergent-associated or detergent-bound molecules that may be present in the aqueous solution, to form an aqueous phase and a non-aqueous phase, for effectively removing the detergent and any detergent-associated or detergent-bound molecules, and sequestering them into the non- aqueous phase. The present composition, system, method and kit may, thus, ideally be used to remove inhibitors from aqueous solutions, i.e., inhibitors that inhibit reactions such as downstream applications including, but not limited to, Polymerase Chain Reaction (PCR, including Reverse Transcriptase-PCR (RT-PCR)), library preparation, and nucleotide sequencing. The aqueous phase may then be used directly for these downstream applications. |