http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017184075-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_437ee9763d95aec48d654f43f7348417 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_015e5ded54d4b564f81a0bfd9fb16e3e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 2016-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_596daa198ed0a051fe1c34af5b4eaca2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4b82b9bec9d177798e747eb82feef21 |
| publicationDate | 2017-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2017184075-A1 |
| titleOfInvention | Detection methods |
| abstract | The present invention relates to a detection method. In particular, it relates to a detection method for detecting the presence and/or absence of plurality of shrimp pathogens in a sample. The method includes the use of polymerase chain reaction (PCR) followed by high resolution melting (HRM) analysis methods. In an aspect of the present invention, there is provided a method for detecting the presence or absence of a plurality of shrimp pathogens in a sample, the sample suspected of containing nucleic acids of at least one shrimp pathogen, the method comprising: (a) exposing the nucleic acids to at least one pair of primers to amplify the nucleic acids, or a portion of each nucleic acid; (b) performing a high resolution melt (HRM) analysis on a double-stranded product comprising the amplicons obtained from the amplification to obtain a melt curve for the sample; and (c) comparing the melt curve with a pre-determined set of parameters, the parameters comprising a plurality of melting temperatures, each melting temperature is associated with a nucleic acid obtained from a shrimp pathogen, and the difference between each melting temperature is at least 1°C, wherein a spike in the melt curve corresponding to the melting temperature associated with a shrimp pathogen indicates the presence of that pathogen in the sample. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114369608-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114369608-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110016523-A |
| priorityDate | 2016-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID226395685 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID6249 |
Total number of triples: 19.