| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3f715ca5afc11b8290f8f916a3326606
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07007
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate |
2017-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd8894a476e664c9f947966ebf97d49d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_313699b21101f811a27071c14637ea51
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ba3ee4d10feb8a5d05f256361d9bea9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_415e896a8395a33a6091e10a4a6a88d3 |
| publicationDate |
2017-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2017148860-A1 |
| titleOfInvention |
Polymerase-template complexes for nanopore sequencing |
| abstract |
The present disclosure provides methods and compositions for enhancing the processivity of a polymerase in catalyzing template-dependent DNA synthesis in high concentrations, more than 100 mM, of salt. Also disclosed are methods and compositions for enhancing the assembly of polymerase-template complex compatible with active DNA synthesis in the presence of levels of nucleotides at 0.8 - 2.2 micormolar and at a high temperature between 35 and 45°C, such as temperatures at or near the melting temperature of the polymerase. The complexes of template and polymerase may be used in nanopore sequencing. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7323534-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111771001-A |
| priorityDate |
2016-02-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |