| abstract |
The present invention relates to the field of biotechnology and provides a method for expressing and preparing a polyvalent multi-specific antibody and immune hybrid protein. The method comprises: preparing, by using a protein intein, a polyvalent multi-specific antibody, or a hybrid immune protein comprising the antibody fused with a cytokine, or an immunotoxin comprising the antibody fused with a protein toxin, or a hybrid immune protein comprising the antibody fused with another active protein. The components of the hybrid protein are respectively expressed in a suitable prokaryotic or eukaryotic cell system, isolated and purified with high-performance affinity chromatography, and then spliced in vitro under an intein splicing condition to prepare the polyvalent multi-specific antibody and the immune hybrid protein products. |