| abstract |
The present invention provides a cell culture medium in which an epithelial stem cell, a cell carcinoma cell or a tissue containing at least one of the aforementioned cells can be cultured in the absence of serum for a long period. The cell culture medium according to the present invention contains: a Wnt agonist composed of a complex of Wnt protein and afamin, which is a substance capable of stabilizing the Wnt protein, and R-spondin; and at least one component selected from the group consisting of a division promoting growth factor, a bone morphogenetic factor (bone morphogenetic protein; BMP) inhibitor, a transforming growth factor-β (TGF-β) inhibitor and a p38 inhibitor. |