abstract |
Disclosed are a nucleotide sequence and the method thereof for controlling an insect infestation, wherein the isolated nucleotide sequence comprises: (a) the polynucleotide sequence as shown in SEQ ID NO: 1, 2 or 3; or (b) a polynucleotide sequence hybridized with the polynucleotide sequence defined in (a) under stringent conditions; or (c) a polynucleotide sequence having at least 80% identity to the polynucleotide sequence defined in (a); or (d) a polynucleotide sequence of at least 19 consecutive nucleotides of the polynucleotide sequence defined in (a), wherein a Hemiptera insect pest ingests a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence, thus inhibiting the growth of the Hemiptera insect pest; or (e) a complementary sequence of the polynucleotide sequence as defined in the above-mentioned (a), (b), (c) or (d). The three target sequences for controlling a Hemiptera insect pest of Lygus have the advantages of high efficiency, specificity and convenience, and a low cost. |