| abstract |
The present invention provides a genetic modification non-human organism where Cas9 expression is higher and a plurality of different genes, or a plurality of sites within the same gene, can be simultaneously edited with high efficiency. This genetic modification non-human organism has at least three copies of the gene encoding Cas9 (CRISPR-associated 9) within the nuclear genome. These egg cells are derived from a genetic modification non-human organism having a nuclear genome into which at least three copies of a gene encoding CAS9 have been introduced. These fertilized eggs are obtained by allowing fertilization to occur between the egg cells and sperm derived from an organism of the same species. This method for modifying a target gene comprises a step for introducing guide RNA to cells derived from the genetic modification non-human organism, the egg cells, or the fertilized eggs. |