abstract |
mRNAs containing an exogenous open reading frame (ORF) flanked by a 5' untranslated region (UTR) and a 3' UTR is provided, wherein the 5' and 3' UTRs are derived from a naturally abundant mRNA in a tissue. Also provided are methods for identifying the 5' and 3' UTRs, and methods for making and using the mRNAs. |