http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017088750-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a9cb516da66bb6d8cbf46d373c40200 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1fc18edc5b85459541e3daa15edeb56f |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-106 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2016-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57ad9095b8cb5dbb752e943dbe4ca00f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88f6f98343366e92682c87243314b919 |
| publicationDate | 2017-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2017088750-A1 |
| titleOfInvention | Method and kit for deviation amplification of target nucleic acid sequence in sample |
| abstract | Provided is a method for deviation amplification of a target nucleic acid sequence having a mutation relative to a wild type form in a sample, comprising: providing a single-stranded closed sequence complementary to at least a part of a sense strand and/or antisense strand of the wild-type form of the target nucleic acid sequence, wherein one or more locked nucleic acid substitutions are comprised in the closed sequence; providing a PCR reaction system comprising the sample, the closed sequence, and a forward and reverse primer designed according to the target nucleic acid sequence and performing a cyclic amplification reaction, wherein the denaturation steps comprise: raising the temperature to a sufficiently high temperature, contacting the closed sequence with the nucleic acid in the sample, and then cooling to a first temperature to make the closed sequence, the target nucleic acid sequence and the wild-type forming of the target nucleic acid sequence form a duplex, wherein the first temperature is higher than the annealing temperature of the primer and the template, and then raising the temperature to a second temperature of approximately 1-5ºC lower than the melting temperature (T m ) of the duplex formed by the closed sequence and the wild type of the target nucleic acid sequence, and higher than the T m of the wild-type form duplex of the target nucleic acid sequence. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107164519-A |
| priorityDate | 2015-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.