| abstract |
In various embodiments devices and methods for the detection and/or quantification of clinically relevant pathogens ( e.g ., bacteria, fungi, viruses, etc.) are provided. In certain embodiments the device comprises a lateral-flow assay that detects the bacterium at a concentration of less than about 6 x 10 6 cells/mL, less than about 3 x 10 6 cells/ml, less than about 1x10 6 CFU/mL, or less than about 50 µg/mL. In certain embodiments the device comprises an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a lateral-flow assay (LFA). In certain embodiments the device comprises a flow-through system comprising a concentration component comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a detection component disposed beneath said concentration component. |