http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017038107-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_49496fec25b22788b50d87dc0f48c692 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P41-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P41-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-22 |
| filingDate | 2016-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2eb93890b7e56b87a7f3eefd8a6d6000 |
| publicationDate | 2017-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2017038107-A1 |
| titleOfInvention | Hasa-redoxin complex having oxidation reaction, and method for producing same |
| abstract | Provided are: a HasA-redoxin complex (a heme iron active-type HasA) which can asymmetrically oxidize only one of enantiomers of a secondary alcohol without the need of adding a coenzyme and has an asymmetric oxidization activity in an aqueous solvent in the presence of oxygen, and an immobilized form of the HasA-redoxin complex; methods respectively for producing the HasA-redoxin complex and the immobilized form of the HasA-redoxin complex; and a production method relating to the coating of the protein complex with a polymeric compound.nMethods respectively for producing a HasA-redoxin complex (a heme iron active-type HasA) having an asymmetric oxidation reaction and an immobilized form thereof. The methods are characterized by comprising: a first step comprising a treatment for encapsulating a plant-originated water-soluble crude protein in a gel, a treatment for oxidizing the gel with air, and a treatment for eluting a HasA-redoxin complex from the gel into an aqueous solution; and a second step comprising a treatment for applying a gravity to the aqueous HasA-redoxin complex solution to concentrate and precipitate the HasA-redoxin complex, a treatment for re-dissolving the HasA-redoxin complex precipitates in an aqueous solution to allow the HasA-redoxin complex and a polymeric compound to co-exist homogeneously, and dehydration/drying of the co-existing aqueous solution to produce a polymeric compound-coated HasA-redoxin complex. The methods are also characterized in that HasA is allowed to express using a transformant (a microorganism) containing HasA originated from Pseudomonas fluorescens , and then the HasA is agitated and oxidized with air in an oxygen-containing aqueous solution, thereby producing a HasA-redoxin complex (a heme iron active-type HasA). |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110283839-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110283839-A |
| priorityDate | 2015-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.