| abstract |
A bispecific nano-antibody, including: (a) a first binding domain having specific binding with human CEA, the first binding domain including an anti-human CEA VHH, and (b) a second binding domain having specific binding with human CD16, the second binding domain including an anti-human CD16 VHH. The antibody molecule is capable of soluble expression within a prokaryotic expression system, is beneficial for subsequent separation and purification, has relatively good heat stability, and is highly soluble. In addition, the antibody fragments used in the present invention come from camelid heavy-chain antibody variable region sequences, have high binding affinity with antigens, have a relatively high homology with human immunoglobulin heavy-chain variable region sequences, and have weak antigenicity. |