| abstract |
A three-dimensional microfluidic device is described, including: a plurality of porous, hydrophilic cellulosic layers each comprising one or more hydrophilic regions and/or hydrophilic channels embedded in the porous, hydrophilic cellulosic layer; wherein the hydrophilic channel is fluidically connected to the hydrophilic region; the hydrophilic regions comprise a sample deposition zone, one or more assay zones in fluidic communication with the sample deposition zone; and a buffer deposition zone; and the assay zone comprises one or more reagents embedded therein selected from the group consisting of antigens and antibodies; and a valve layer comprising a valve switchable from a first position where the buffer zone and the assay zone are not in fluidic communication, to a second position where the buffer zone and the assay zone are in fluidic communication. |