| abstract |
This disclosure relates to CRISPR-associated proteins from Francisella (CPF1), variants, fusions, and nucleic acid complexes related thereto. In certain embodiments, the disclosure relates to recombinant vectors encoding a CPF1 and variants thereof. In certain embodiments, the disclosure relates to CPF1 and guide RNA complexes for use in targeted binding and/or cutting of nucleic acids, e.g., DNA, mRNA, or viral RNA. In certain embodiments, the disclosure contemplates genome editing using CPF1 and nucleic acid complexes disclosed herein. |