| abstract |
The invention is directed to a method for manufacturing a negatively charged supported lipid bilayer. Said method comprises the steps of preparing a formulation comprising at least three lipids (1,2-dioleoyl-sn-glycero-3-phospho-L- serine (DOPS), cholesterol and at least one lipid different from DOPS and cholesterol) dissolved in a first solvent, of evaporating said first solvent, of adding an aqueous formulation of mesoporous silica nanoparticles, of performing an ultra- sonication and of performing a centrifugation. Said method is remarkable in that the number of equivalents of cholesterol relative to one equivalent of DOPS is comprised between 2.30 and 2.70. The invention is further directed to negatively charged supported lipid bilayer on a mesoporous silica nanoparticle comprising cholesterol, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) and at least one lipid different from DOPS and cholesterol. |