| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_856a9a7918c9c8166ec5dd6de6f99235
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5098eff6d67391cd857fae0aa3174bcb |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-92 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 |
| filingDate |
2016-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5b361856ffd355d08f98cb07eca5006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef01050fbd2511826241ab4e2f1128f8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a16268c26fce20f7065c18ca5e637d45
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e6702c77eb6f78dc50ec43a3367200f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fbac8a611651b64e7739826c1fd1ad5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8947e90efcc11eec18ed1e636022b4d4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5236f0ec887cf01418098760504cafea
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0247467151bb58c3168524b9cfc9c144 |
| publicationDate |
2016-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2016164596-A2 |
| titleOfInvention |
Methods for selecting enzymes having lipase activity |
| abstract |
Provided herein are methods and means for enhancing lipase activity. The system makes use of an emulsion for in vitro compartmentalization of a library of synthetic compounds which have a polynucleotide linked to a lipase substrate (e.g., a triglyceride). Expressed polypeptides having greater lipase activity will preferentially hydrolyze the substrate from the linked polynucleotide. Genes encoding polypeptides having less lipase activity will remain linked to the substrate and may be removed to enrich the library for more active variants. Also described are synthetic compounds and emulsions which can be used in the methods. |
| priorityDate |
2015-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |