http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016156469-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b7c92124a491c7d15c28385198cd801d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2016-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c901100ec261dfa7902ddf302814523 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_925cc4ed3c0551e8aea865d05ac14768 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a443664a4989f584bae5ff4c34f6b1e3 |
| publicationDate | 2016-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2016156469-A1 |
| titleOfInvention | Genome architecture mapping on chromatin |
| abstract | The present invention relates to the field of analysis of the three-dimensional structure of the genome, i.e., for genome architecture mapping on chromatin (GAM-ch). The invention provides a method of determining interaction of a plurality of nucleic acid loci in a compartment comprising nucleic acids, such as the cell nucleus, comprising separating nucleic acids from each other depending on their interaction in the compartment by crosslinking nucleic acids with each other directly or indirectly, fragmenting the nucleic acids of the compartment to obtain fragments and/or cross-linked complexes of fragments, and dividing the fragmented nucleic acids to obtain a collection of fractions such that every fraction contains, on average, less than one copy of every locus; determining the presence or absence of the plurality of loci in said fractions; and determining the co-segregation of said plurality of loci in the fractions. Co-segregation may then be analysed with statistical methods to determine interactions. The method can be used e.g., for identifying the frequency of interactions across a cell population between a plurality of loci; and mapping loci and/or genome architecture, e.g., in the nucleus, an organelle, a microorganism or a virus; identification of regulatory regions directing expression of a specific gene through spatial contacts; identifying the spatial contacts between loci that depend on their co-association with specific protein(s) or RNA and/or diagnosing a disease associated with a disturbed co- segregation of loci. Chromatin immunoprecipitation (ChIP) can be combined with the method of the invention. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018045137-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022084528-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3988669-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111727248-A |
| priorityDate | 2015-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 392.