| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c10e092da962c0b38c3d86e0ac840882 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-137
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-32 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2016-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1ec74039ff81fef2174725f02f240c3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7baefdc77db3da14adc8dea88e442f06 |
| publicationDate |
2016-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2016147004-A1 |
| titleOfInvention |
Isolation of nucleic acids |
| abstract |
Methods of isolating nucleic acids comprising DNA from biological material are disclosed. The methods comprise a lysis step using an aqueous composition which comprises lithium at a concentration of 0.05 to 1.0M, a chelating agent, and a surfactant to produce a lysed composition. The lysed composition is treated with a solid support that is capable of immobilising DNA in the presence of a dissolved chaotropic agent at a concentration of 0.05 to 2M and 25% to 60% by volume of a C 1-3 alkanol. The support is then washed with a first wash solution containing lithium dissolved in a C 1-3 alkanol. Subsequently, the support is washed with a liquid comprising at least 80% by volume of a C 1-3 alkanol. The nucleic acid comprising DNA is then eluted from the support. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108427000-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018063772-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108427000-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020165602-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3985112-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11015186-B2 |
| priorityDate |
2015-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |