| abstract |
A method for producing skeletal muscle progenitor cells from pluripotent stem cells, said method comprising the following steps (1) and (2A) or (2B):(1) a step for culturing the pluripotent stem cells in a liquid culture medium containing a TGF-β inhibitor and a GSK3β inhibitor; (2A) a step for culturing the cells obtained in step (1) in a liquid culture medium containing HGF optionally together with IGF1; or (2B) a step for culturing the cells obtained in step (1) successively (i) in a liquid culture medium containing a TGF-β inhibitor, a GSK3β inhibitor, IGF1, HGF and bFGF, (ii) in a liquid culture medium containing a TGF-β inhibitor, a GSK3β inhibitor and IGF1, and (iii) in a liquid culture medium containing a TGF-β inhibitor, IGF1 and HGF. Further, this method may comprise (3) a step for culturing the cells obtained in step (2A) or (2B) in a liquid culture medium containing a TGF-β inhibitor, IGF1 and serum. |